Genetic diversity analysis of Chinese Leishmania isolates and development of L. donovani complex-specific markers by RAPD

Abstract Background Leishmaniasis is one of the most neglected tropical diseases in world and remains endemic some underdeveloped regions, including western China. The phylogeny classification Chinese Leishmania has not been completely clarified to date, especially within ( L. ) donovani complex, although phylogenetic analyses based on a series gene markers have performed. More analytic methods data are still needed. Random amplified polymorphic DNA (RAPD) technology can sensitively identify slight intraspecific differences, it powerful tool seek species-specific markers. This work attempted isolates from diverse geographic regions at genomic level. Meanwhile, specific complex were also developed by RAPD. Methods RAPD was applied 14 3 WHO reference strains. sites amplification transformed into matrix, which genetic similarity calculated, UPGMA dendrogram constructed analyse diversity these isolates. loci TA-cloned, sequenced converted sequence characterized region (SCAR) markers, validated preliminarily 17 available strains this study analysed bioinformatics. Results cluster showed that three sp. SC10H2, SD GL clustered together apart others, clearly divided two clades, Cy, WenChuan 801 formed subclade. Three SCAR i.e., 1-AD17, 2-A816 3-O13, successfully obtained study. Through bioinformatic analyses, Marker 1-AD17 may more specificity for PCR detection VL, 3-O13 potential encode protein. Conclusions results verified undescribed species causing visceral leishmaniasis (VL) China unique clade distinguished revealed there differentiation among . identification -specific help provide foundation future research attempting develop new diagnostic VL functions.